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4x protein loading buffer  (LI-COR)


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    Structured Review

    LI-COR 4x protein loading buffer
    4x Protein Loading Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 96/100, based on 486 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protein+loading/PROTEIN+LOADING+BUFFER/bio_rxiv__64898__2026__10__03__756445-141-4-8
    Average 96 stars, based on 486 article reviews
    4x protein loading buffer - by Bioz Stars, 2026-10
    96/100 stars

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    Protein Concentration:

    Article Title: A Phenotypic High-Throughput Screen Identifies Small Molecule Modulators of Endogenous RGS10 in BV-2 Cells
    Article Snippet: Protein concentration was determined using the Pierce BCA Protein Assay (#23225). .. Protein concentration was adjusted in each sample to allow for equal protein loading, and sample buffer (Li-Cor; Lincoln, NE; #928-40004) was added before loading samples on the gel. .. Equal amounts of proteins were loaded onto a 12% SDS/PAGE gel and resolved at 160 V and 0.4 A for 1 h. Proteins were transferred to an Immobilon-P PDVF membrane (EMD Millipore, Burlington, MA; #IPVH00010) for 2 h at 160 V, 0.4 A.

    Article Title: A Phenotypic High-Throughput Screen Identifies Small Molecule Modulators of Endogenous RGS10 in BV-2 Cells.
    Article Snippet: Protein concentration was determined using the Pierce BCA Protein Assay (#23225). .. Protein concentration was adjusted in each sample to allow for equal protein loading, and sample buffer (Li-Cor; Lincoln, NE; #928-40004) was added before loading samples on the gel. ..

    Staining:

    Article Title: Loss of O-GlcNAcylation in cardiac myocytes triggers the integrated stress response, contributing to heart failure
    Article Snippet: Quantification of band intensities was performed with Image Studio Lite (LI-COR). .. Normalization for protein loading was performed based on intensities obtained by staining the membranes with REVERT 700 Total Protein Stain (Cat. No. 926-11010, LI-COR). ..

    Article Title: Deep homology and design of proteasome chaperone proteins in Candida auris
    Article Snippet: Blots were washed with PBS-T and incubated with 1:15000 dilution anti-mouse IgG IRDye-800CW secondary antibody (LI-COR Biosciences, 926-33210) before detecting signals using a LI-COR Odyssey scanner. .. Protein levels were normalized to total protein loading using Revert 700 total protein stain (LI-COR Biosciences, 926-11011). .. Proteasome α-subunits were detected using a primary antibody specific to a conserved peptide in the 20S alpha proteasome proteins Pre1, Pre2, Pre3, Pre5, Pre6, and Pre7 at a 1:3000 dilution in blocking solution (AbCam, antibody ID: AB_2171376).

    Article Title: A modular toolbox for in cellulo screening of small molecule inhibitors targeting chromatin reader domains
    Article Snippet: Protein detection was done using species-specific antibodies conjugated to horseradish peroxidase and Pierce® Peroxidase IHC Detection Kit (Thermo Scientific). .. Total protein loading was assessed using RevertTM 700 Total Protein Stain (LI-COR Biosciences, 926-11011) according to the manufacturer’s instructions. ..

    Article Title: Loss of O-GlcNAcylation in cardiac myocytes triggers the integrated stress response, contributing to heart failure.
    Article Snippet: Quantification of band intensities was performed with Image Studio Lite (LICOR). .. Normalization for protein loading was performed based on intensities obtained by staining the membranes with REVERT 700 Total Protein Stain (Cat. No. 926-11010, LI-COR). ..

    Article Title: Supporting Information Deoxyguanosine-linked bifunctional inhibitor of SAMHD1 dNTPase activity and nucleic acid binding
    Article Snippet: Membrane was imaged with LI-COR Odyssey CLx imager. .. Protein loading was determined by staining for GAPDH (LI-COR 926-42216) using a donkey anti-rabbit IRDye 680CW antibody (LI-COR 926- 68073) and imaged as described above. ..

    Control:

    Article Title: Pharmacokinetics and efficacy of tank-water administered BRAF-inhibitor dabrafenib in a zebrafish model of BRAF-mutant melanoma
    Article Snippet: To control for protein loading, the membranes were stripped with stripping buffer (25 mM glycine-HCl, 1% SDS pH 2) for 20 min and blocked with 5% fat-free milk in TBST before incubating with monoclonal anti-beta-actin antibody (1:1000, cat# A2228, Sigma-Aldrich) and IRDye 680RD Donkey anti-Mouse (1:10 000, LI-COR). .. To control for protein loading, the membranes were stripped with stripping buffer (25 mM glycine-HCl, 1% SDS pH 2) for 20 min and blocked with 5% fat-free milk in TBST before incubating with monoclonal anti-beta-actin antibody (1:1000, cat# A2228, Sigma-Aldrich) and IRDye 680RD Donkey anti-Mouse (1:10 000, LI-COR). ..

    Stripping Membranes:

    Article Title: Pharmacokinetics and efficacy of tank-water administered BRAF-inhibitor dabrafenib in a zebrafish model of BRAF-mutant melanoma
    Article Snippet: To control for protein loading, the membranes were stripped with stripping buffer (25 mM glycine-HCl, 1% SDS pH 2) for 20 min and blocked with 5% fat-free milk in TBST before incubating with monoclonal anti-beta-actin antibody (1:1000, cat# A2228, Sigma-Aldrich) and IRDye 680RD Donkey anti-Mouse (1:10 000, LI-COR). .. To control for protein loading, the membranes were stripped with stripping buffer (25 mM glycine-HCl, 1% SDS pH 2) for 20 min and blocked with 5% fat-free milk in TBST before incubating with monoclonal anti-beta-actin antibody (1:1000, cat# A2228, Sigma-Aldrich) and IRDye 680RD Donkey anti-Mouse (1:10 000, LI-COR). ..



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